Shared Equipment
Shared Equipment
FlexCell 6000 Strain Unit
This system is designed to expose cells in culture to a mechanical environment (strain). The system also includes an adapter to image live cells in real time on an inverted microscope.
Cells are being grown in culture on 6 well plates (purchased from FlexCell) with flexible membrane surfaces. A CPU controlled vacuum controls the magnitude and frequency of membrane stretch.
The system can only be used for microscopic imaging of for cell culture exposures. Cells cannot be imaged while cells are being stimulated in culture.
Contact
 | Dr. Heather Drummond Office: G351-02 (601) 601-984-1812 |
QuantStudio 5 Real-Time PCR System
The QuantStudio 5 Real-Time PCR System detects changes in gene expressions as low as 1.5-fold.
The system supports a broad range of genomic applications, such as analyses of gene expression, microRNAs and noncoding RNAs, copy number variation, drug metabolism enzymes, and protein expression; SNP genotyping; and mutation dection.
The QuantStudio system has a 96-welllock format feature which allows for independent temperature control in each zone allowing multiple temperature optimizations in the same run. This system also allows for use of probe-based assays and intercalating dyes.
Contact
 | Dr. Ana Omoto Office: G361 Phone: (601) 984-1823 |
GentleMACS Octo Dissociator with Heaters
The gentleMACS Octo Dissociater with Heaters is a benchtop instrument for automated and standardized tissue dissociation or homogenization of up to eight samples. It is equipped with eight individual heating units and optimized and ready-to-use gentleMACS.
Programs the gentleMACS Octo Dissociater with Heaters offers a fully automated workflow for tissue dissociation. In addition, the intrument allows the user to create user-defined programs for almost any biological material. All eight positions can be operated independently.
Contact
 | Dr. Jussara doCarmo Office: G261 Phone: (601) 984-4353 |
Thermo Sorvall X1 Pro with Cell Culture Package
The Sorvall X1 Pro is a general use centrifugation package for the routine harvesting and passaging of mammalian cell cultures.
The cell culture package includes inserts that can accommodate 15mL and 50mL centrifuge tubes and biocontainment aerosol lids to allow for processing of cells and tissues that require higher levels of biosafety measures.
Contact
 | Dr. Eric George Office: G155 Phone: (601) 984-5299 |
Seahorse Extracellular Flux Analyzer XFe96
The Seahorse Extracellular Flux Analyzer can measure glycolysis and mitochondrial oxygen consumption in a 96-well format. Standarized kits include glycolysis, mitochondrial oxidative phosphorylation, glucose oxidation, fatty acid oxydation, etc. Assays can be customized for drug delivery or alternative metabolic pathways (i.e. ketone and lactate oxidation). Inputs include cultured cells, isolated mitochondria, or live tissue cultures.
Contact
 | Dr. Alan Mouton Office: G258 Phone: (601) 984-1819 |
NanoSight Pro
NanoSight Pro (Nanoparticle Tracking Analysis, NTA) is an advanced platform for the characterization of nanoparticles in liquid suspension. It utilizes Nanoparticl Tracking Analysis (NTA) technology to visualize, track, and analyze individual particles in real time based on their Brownian motion.
By illuminating particles with a laser and capturing scattered light via a high-sensitivity camera, the NanoSight Pro calculates particle size and concentration using the Stokes-Einstein equation. This allows accurate measurement of particle size distributions typically ranging from ~10 to 1,000 nm, making it particularly well suited for the analysis of extracellular vesicles, viruses, lipsomes, protein aggregates, and synthetic nanoparticles.
Contact
| Dr. Ana Omoto Office: G361 Phone: (601) 984-1823 |
Eppendorf Centrifuge 5702R
Eppendorf Centrifuge 5702R is a benchtop refrigerated centrifuge used for separation of biological samples by centrifugal force while maintaining controlled low temperature to preserve sample integrity. Typically, it is used for pelleting cells from culture media, separating plasma or serum from blood samples, spinning down protein, DNA, RAN samples, processing tissue homogenates and subcellular fractions, and routine centrifugation of tubes and plates. The included rotor allows compatibility with common tube formats, making it suitable for daily laboratory workflows.
Contact
| Dr. Jussara doCarmo Office: G261 Phone: (601) 984-4353 |
Beckman Coulter XE-100-IVD Ultracentrifuge
Beckman Coulter Optima XE-100-IVD is a high speed analytical ultracentrifuge used for separating particles in complex biological samples at very high centrifugal forces.
Typical uses include:
- Fractioning subcellular components (e.g. membranes, organelles, EVS)
- Purifying viruses, virus-like particles, exosomes, and large protein complexes
- Separating lipoproteins or macromolecules by density
- Research requiring high g-force separations not achievable with standard lab centrifuges
Its ultracentrifugation capability enables resolution of very small particles and macromolecular assemblies, making it essential for advanced biochemical, structural biology, and clinical research workflows.
Contact
| Dr. Jussara doCarmo Office: G261 Phone: (601) 984-4353 |  | Dr. Josh Speed Office: G558 Phone: (601) 815-4582 |
AD Instruments Isolated Muscle and Nerve Stimulator
The AD Instruments Isolated Muscle and Nerve Stimulator is a system that consists of two software-controlled AD Instruments Muscle and Nerve Stimulators, which are designed to work with a C range of constant-current
stimulations at a compliance voltage of 100 V. Depending on the experimental requirements and protocols, both stimulators may be used for in vitro tissue (nerve, vessel, muscle) stimulation.
Stimulator HC is ideal for field or larger current in vitro stimulations, such as stimulation of larger isolated muscles. The stimulus isolator is ideal for direct or smaller current in vitro stimulations, such as isolated nerve stimulation and it also provides isolated stimulation, which allows in vitro nerve and muscle stimulation of animals. Both stimulators are powered by their own Stimulatory Power Supply (115/230V:12VAC) and controlled by LabChart software via two PowerLab connections. This system is ideal for evaluating the effects of metabolic diseases like obesity and diabetes as well as the effect of aging on muscle performance and endurance. This system allows for direct or field stimulation of in vitro tissues samples or in vitro studies involving nerve and muscle stimulation.
Contact
 | Dr. David Stec |
Azure Sapphire Biomolecular Imager
The Azure Sapphire Biomolecular imager allows for Multiplex Westerns Quantitative Westerns, Total protein normailzation, In-cell Westerns Cell-based assays, Multi-well plate imaging Protein arrays, Microarrays and Immunohistochemistry.
The Sapphire FL and Sapphire FL+ enable scanning at wavelengths from 375 nm to 900 nm, allowing detection of 3 separate protein targets by Western blot. The enhanced sensitivity of the Sapphire Biomolecular imager allos for femtogram detection of proteins labeled with common fluroescent dyes.
Contact
| Dr. David Stec |
Azure 300 Chemiluminescent/DNA Imaging System
The Azure 300 Chemiluminescent/DNA imaging system allows for UV, color imaging, blue-excited DNA dyes, silver-stain, and Coomassie gel imaging.
Using the preset protocols allows for a comprehensive list of typical detections, or generate your own custom protocols combining any excitation source, emission filter, image overlay, lens aperature, exposure time, focus, distance to the camera, and resolution.
Contact
| Dr. David Stec |
PAMStation 12, Model 2
The PAMStation 12, Model 2 is an instrument that measures real-time kinase activity from all types of fresh or frozen biological samples including clinical biopsies. The PAMStation 12 uses specific PAMChips to measure either Serine/Threonine (STK) or Tyrosine phosphorylation (PTK) activity using unique technology in which a general antibody is able to bind to phosphorylated targets (144 for the STK and 196 for the PTK) spotted on the PamChips. The PAMStation 12 is ideal for signaling and disease model characterization. It can also be used for biomarker treatment.
The PAMStation 12 uses "chip" technology which is more high-throughput than similar measurements potentially made by mass-spectrometry. The PAMStation Core will provide expertise in the design of experiments, the preparation of cell/tissue lysates for analysis, and the running of the PAMChips. PAMStation data can be analyzed using the Tercen software provided by PAMGene (https://bionavigator.pamgene.com/). Tercen software allows for the quality control, normalization, kinase analysis, and pathway analysis of PAMStation PTK and STK data. PAMStation users are responsible for the analysis of their data.
Contact
| Dr. David Stec |
Oroboros O2K fluorespirometry (NextGen-O2k all-in-one)
The complete experimental system for high resolution fluorespirometry, redox biology and PhotoBiology:
enabling simultaneous monitoring of oxygen consumption together with coenzyme Q redox state, NAD(P)H redox state, H2O2 production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+uptake capacity (CaG), ATP production (MgG) or photobiology measurements from isolated mitochondria, intact and permeabilized cells, premeabilized tissue and tissue homogenates from small biopsies.
Contact
 | Dr. Zhen Wang Office: G262 Phone: 601-984-1250 |
Seahorse XFe24 Analyzer
Agilent Seahorse XFe24 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 24-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate, and together these measurements provide a systems-level view of cellualar metabolic function in cultured cells and ex-vivo samples.
Contact
| Dr. Zhen Wang Office: G262 Phone: 601-984-1250 |
KEYENCE All-in-One Fluorescence Microscope
The BZ-X Series is an all-in-one fluorescence microscope equipped with advanced observation capabilities, a fully-monitored control system for instant target axquisition, and advanced imaging and analysis functions. With a built-in darkroom and an installation area of ~1' x 2' . The BZ-X inverted microscope can be set up in any location for optimal testing efficiency. The high-sensitivity, cooled CCD camera can be switched between monochrome and color imaging, and supports fluorescence, brightfiled imaging. The large motorized stage can quickly move to the desired observation location, while high speed auto-focusing and automatic capture conditions allow any user to easily capture publication-quality images.
Contact
| Dr. Zhen Wang Office: G262 Phone: 601-984-1250 |